6GN5

CRYSTAL STRUCTURE OF HUMAN GRAMD1C START DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97793
Spacegroup name	P 1 21 1
Unit cell lengths	31.320, 73.850, 45.340
Unit cell angles	90.00, 99.31, 90.00

Refinement procedure

Resolution	38.267 - 1.410
R-factor	0.1837
Rwork	0.182
R-free	0.20520
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.700	44.700	1.450
High resolution limit [Å]	1.410	6.310	1.410
Rmerge	0.056	0.039	0.864
Rmeas	0.061	0.042	0.938
Number of reflections	39116	458	2817
<I/σ(I)>	14.76	40.61	1.68
Completeness [%]	99.8	99.6	97.6
Redundancy	6.69		6.38

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	40% MPD 10% PEG8000 0.1M HEPES PH 7.0