6GMM
Crystal structure of Helicobacter pylori adhesin LabA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.860, 59.860, 264.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.537 - 2.060 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zh7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.537 | 2.093 |
| High resolution limit [Å] | 2.056 | 2.056 |
| Rmerge | 0.242 | 1.516 |
| Number of reflections | 31169 | 1558 |
| <I/σ(I)> | 8.1 | 1.9 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 12.3 | 11 |
| CC(1/2) | 0.999 | 0.599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 28 % PEG 2000 MME, 0.1 M Bis-Tris pH 6.5 |
