6GMA

Crystal structure of the FIP200 C-terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 2
Unit cell lengths	92.045, 187.166, 55.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.758 - 3.200
R-factor	0.2693
Rwork	0.267
R-free	0.29480
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.758	37.758	3.360
High resolution limit [Å]	3.170	9.340	3.170
Rmerge	0.070	0.023	1.989
Rmeas	0.081	0.027	2.303
Number of reflections	16476	672	2624
<I/σ(I)>	11.02	40.39	0.56
Completeness [%]	97.1	89.6	98.4
Redundancy	3.657	3.109	3.778
CC(1/2)	0.999	0.999	0.239

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES pH 6.0, 1.0 M LiCl, 14% PEG 6000