6GM7

[FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii,variant E144A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.8729
Spacegroup name	P 32 2 1
Unit cell lengths	70.910, 70.910, 154.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.107 - 2.140
R-factor	0.1916
Rwork	0.190
R-free	0.22680
RMSD bond length	0.003
RMSD bond angle	0.712
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.120	48.107	2.200
High resolution limit [Å]	2.140	9.570	2.140
Rmerge	0.165	0.034	0.805
Number of reflections	25469
<I/σ(I)>	9.7	38.2	2.03
Completeness [%]	99.4	96.8	99.8
Redundancy	6.84
CC(1/2)	0.993	0.999	0.476

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	20% PEG 4000, 0.4 M NaCl,0.1 M MES