6GM5
[FeFe]-hydrogenase HydA1 from Chlamydomonas reinhardtii,variant E141A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972958 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.620, 70.620, 154.910 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.999 - 1.450 |
R-factor | 0.1668 |
Rwork | 0.165 |
R-free | 0.19690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lx4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.224 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.000 | 47.999 | 1.490 |
High resolution limit [Å] | 1.450 | 6.480 | 1.450 |
Rmerge | 0.079 | 0.043 | 1.079 |
Number of reflections | 79955 | ||
<I/σ(I)> | 22.11 | 60.72 | 2.24 |
Completeness [%] | 99.8 | 99.6 | 97.2 |
Redundancy | 18.38 | ||
CC(1/2) | 1.000 | 1.000 | 0.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 11% PEG 4000, 0.5 M NaCl,0.1 M MES |