6GKX

Crystal structure of the R-type bacteriocin tube protein CD1364 from Clostridium difficile in the pre-assembled state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 43 21 2
Unit cell lengths	36.323, 36.323, 216.813
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.824 - 1.500
R-factor	0.1928
Rwork	0.192
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2guj
Data reduction software	XDS
Data scaling software	Aimless (0.6.3)
Phasing software	PHENIX
Refinement software	PHENIX (dev_3112)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.360	43.360	1.530
High resolution limit [Å]	1.500	8.220	1.500
Rmerge	0.083	0.041	2.290
Rmeas	0.085	0.042	2.335
Rpim	0.017	0.010	0.455
Number of reflections	24715	227	1194
<I/σ(I)>	19.7
Completeness [%]	100.0	99.8	100
Redundancy	24.8	17.4	25.9
CC(1/2)	1.000	1.000	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 0.2 M ammoniumchloride