6GJO
Crystal Structure of Glycogen Synthase Kinase-3 beta in Complex with BI-91BS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.712, 67.484, 67.787 |
| Unit cell angles | 77.51, 77.56, 89.21 |
Refinement procedure
| Resolution | 19.960 - 2.910 |
| R-factor | 0.218 |
| Rwork | 0.217 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 3.060 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmeas | 0.156 | 0.367 |
| Number of reflections | 25318 | 2479 |
| <I/σ(I)> | 1.9 | 3.1 |
| Completeness [%] | 68.2 | 52.4 |
| Redundancy | 1.5 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.1 | 277 | 100 mM Tris Acetate pH 8.1, 125 mM NaCl, 15-20% PEG8K |
