6GJE

Structure of the Amnionless(20-357)-Cubilin(36-135) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	I 2 2 2
Unit cell lengths	70.280, 158.840, 237.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.511 - 2.300
R-factor	0.2119
Rwork	0.211
R-free	0.23040
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.726
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.400
High resolution limit [Å]	2.300	2.300
Number of reflections	59436
<I/σ(I)>	19.53
Completeness [%]	100.0
Redundancy	13.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	1.5 M magnesium sulphate 0.1 M MES pH 5.5