6GIX
Water-soluble Chlorophyll Protein (WSCP) from Lepidium virginicum (Mutation L91P) with Chlorophyll-b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-08-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.860, 82.130, 122.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.800 |
| R-factor | 0.21602 |
| Rwork | 0.214 |
| R-free | 0.25927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dre |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.428 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.730 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 18489 | 1336 |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 15 | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M Na/K Phosphate, 3.2M Ammonium sulfate, 5% Sucrose |
