6GIR

Arabidopsis thaliana cytosolic seryl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	I 1 2 1
Unit cell lengths	92.127, 45.607, 103.548
Unit cell angles	90.00, 97.74, 90.00

Refinement procedure

Resolution	30.280 - 2.343
R-factor	0.21463
Rwork	0.211
R-free	0.27845
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qne
RMSD bond length	0.011
RMSD bond angle	1.505
Data reduction software	CrysalisPro (1.171.38.43)
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.280	2.440
High resolution limit [Å]	2.343	2.343
Rmerge	0.127
Number of reflections	16605
<I/σ(I)>	5.29
Completeness [%]	96.1
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Hanging drop vapor diffusion method; mixture of 4 uL 3.9 mg/mL protein solution, 2 uL of precipitating solution (0.2 M MgCl2, 0.1 M Tris pH 7, 30%(w/v) PEG 4000) and 1uL of additive solution (0.6 M MgCl2) at 291 K.