6GHZ

Structure of Lytic Transglycosylase MltE mutant Y192F from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-19
Detector	PSI PILATUS 6M
Wavelength(s)	0.979210
Spacegroup name	C 2 2 21
Unit cell lengths	120.028, 181.339, 35.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.380 - 2.330
R-factor	0.23718
Rwork	0.234
R-free	0.30417
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y8p
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.380	2.410
High resolution limit [Å]	2.330	2.330
Rpim	0.024
Number of reflections	17052
<I/σ(I)>	11.1
Completeness [%]	99.7
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	8.4	291	26% Polyethylene Glycol 4000, 0.2 M magnesium chloride and 0.1 M TRIS HCl (pH 8.4)