Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.395, 64.786, 98.949 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.320 - 2.000 |
| R-factor | 0.2265 |
| Rwork | 0.222 |
| R-free | 0.26920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w7v |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.681 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.320 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.157 | |
| Number of reflections | 10733 | |
| <I/σ(I)> | 7.42 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M L-Proline, 0.1 M HEPES, pH=7.5, PEG 3350 |
