6GHS

Modification dependent TagI restriction endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	294
Detector technology	CCD
Collection date	2018-02-16
Detector	Bruker Platinum 135
Wavelength(s)	1.54
Spacegroup name	P 41 21 2
Unit cell lengths	72.963, 72.963, 207.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.920
R-factor	0.19389
Rwork	0.192
R-free	0.22309
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3clz 5mkw
RMSD bond length	0.008
RMSD bond angle	1.338
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.020
High resolution limit [Å]	2.920	2.920
Rmerge	0.239	0.698
Number of reflections	12496
<I/σ(I)>	4.38	1.2
Completeness [%]	96.1	96.2
Redundancy	3.23	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	7.5	294	A5 Morpheus Buffer (10% w/v PEG 20 000, 20% v/v PEG MME 550, 0.03 M MgCl2, 0.03 M CaCl2, 0.1 M MOPS/HEPES-Na pH 7.5)