6GHO
Crystal structure of Spx in complex with YjbH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.100, 85.340, 109.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.670 - 1.790 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.22140 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.776 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.700 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.043 | |
| Rmeas | 0.047 | 0.943 |
| Number of reflections | 46753 | 4619 |
| <I/σ(I)> | 20.43 | 1.91 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.1 | 5 |
| CC(1/2) | 0.999 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 3350, MgCl2 and Bis-Tris pH 6.0 |
