6GHC

Modification dependent EcoKMcrA restriction endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-11
Detector	MARRESEARCH
Wavelength(s)	1.22021
Spacegroup name	P 21 21 21
Unit cell lengths	82.045, 90.383, 95.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.190 - 2.850
R-factor	0.22144
Rwork	0.218
R-free	0.28272
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.200	3.020
High resolution limit [Å]	2.850	2.850
Rmeas	0.072	1.419
Number of reflections	17013
<I/σ(I)>	20.96	1.82
Completeness [%]	99.1	96.4
Redundancy	11.5	11.4
CC(1/2)	0.999	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	294	0.1 M HEPES-CsOH, pH 7.3, 30% MPD