6GGM
HLA-E*01:03 in complex with the Mtb44 peptide variant: Mtb44*P2-Phe.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.930, 66.041, 72.104 |
| Unit cell angles | 102.30, 101.22, 109.70 |
Refinement procedure
| Resolution | 53.184 - 2.734 |
| R-factor | 0.1822 |
| Rwork | 0.180 |
| R-free | 0.22110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gh1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.692 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.190 | 7.420 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.230 | |
| Number of reflections | 25095 | |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 95.9 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.5 | 2.0 M Ammonium Sulphate, 0.1 M MES, 1mM ZnSO4 |
