6GFU
Crystal structure of an ancient sequence-reconstructed Elongation Factor Tu (node 262)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.07227 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.944, 73.691, 113.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.850 - 2.000 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.23340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.946 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 61.850 | 45.060 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.920 | 2.000 |
| Rmerge | 0.110 | 0.062 | 1.052 |
| Rmeas | 0.116 | 0.065 | 1.111 |
| Rpim | 0.036 | 0.021 | 0.351 |
| Total number of observations | 4054 | 22302 | |
| Number of reflections | 33001 | 449 | 2318 |
| <I/σ(I)> | 13.9 | 36.4 | 2.5 |
| Completeness [%] | 99.1 | 99.6 | 96 |
| Redundancy | 10.4 | 9 | 9.6 |
| CC(1/2) | 0.998 | 0.998 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 10% w/v PEG 4000, 20% v/v glycerol 0.03M of each ethylene glycol (0.3M diethyleneglycol, 0.3M triethyleneglycol, 0.3M tetraethyleneglycol, 0.3M pentaethyleneglycol), 0.1M MOPS/HEPES-Na pH 7.5 |
