6GFM

Crystal structure of the Escherichia coli nucleosidase PpnN (pppGpp-form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97623
Spacegroup name	I 41 2 2
Unit cell lengths	176.920, 176.920, 94.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.700 - 2.770
Rwork	0.181
R-free	0.23190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gfl
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.700	2.869
High resolution limit [Å]	2.770	2.770
Rmerge	0.142	2.704
Rmeas	0.145	2.781
Rpim	0.029	0.642
Number of reflections	19332	1907
<I/σ(I)>	17.45	1.35
Completeness [%]	99.8	99.74
Redundancy	24.8	18.2
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277.15	0.4M KNa tartrate hydrate