6GFB
Structure of the BTB/POZ domain of human 90K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98010 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.340, 70.340, 105.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.770 - 2.080 |
| R-factor | 0.2099 |
| Rwork | 0.208 |
| R-free | 0.24330 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.210 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmeas | 0.120 | 1.591 |
| Number of reflections | 18533 | |
| <I/σ(I)> | 14.46 | 1.42 |
| Completeness [%] | 99.2 | 94.9 |
| Redundancy | 18.6 | 17.2 |
| CC(1/2) | 0.990 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 9% PEG 8000 0.2M zinc acetate 0.1M imidazole pH 6.8 |
