6GF7
Molecular basis of egg coat filament cross-linking: Zn-SAD structure of the partially deglycosylated ZP1 ZP-N1 domain homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.2825 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 75.410, 75.410, 100.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.323 - 2.700 |
| R-factor | 0.227 |
| Rwork | 0.222 |
| R-free | 0.27180 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.723 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_3409: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.323 | 2.820 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmeas | 0.103 | 1.627 |
| Rpim | 0.041 | 0.852 |
| Number of reflections | 15254 | 1896 |
| <I/σ(I)> | 12.4 | 0.9 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 6.1 | 3.4 |
| CC(1/2) | 1.000 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.6 M LiCl, 0.1 M tri-sodium citrate pH 5.5 |
