6GED

Adhesin domain of PrgB from Enterococcus faecalis bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-03
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	32.017, 93.026, 99.097
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.732 - 1.794
R-factor	0.1762
Rwork	0.174
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6EVT
RMSD bond length	0.012
RMSD bond angle	1.152
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.732	1.900
High resolution limit [Å]	1.790	1.790
Rmeas	0.172	1.670
Number of reflections	28379
<I/σ(I)>	14.22	1.63
Completeness [%]	99.3	98.6
Redundancy	13.36
CC(1/2)	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.12 M Ethylene Glycol, 0.1 M Bicine/Tris pH 8.5, 20% (v/v) Glycerol, 10% (w/v) Polythyleneglycol 4000