6GDW
Structure of CutA from Synechococcus elongatus PCC7942 complexed with 2 molecules of Bis-Tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.700, 95.820, 48.750 |
| Unit cell angles | 90.00, 97.11, 90.00 |
Refinement procedure
| Resolution | 47.910 - 1.800 |
| R-factor | 0.20499 |
| Rwork | 0.203 |
| R-free | 0.24371 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gdv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.646 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.105 | |
| Rpim | 0.018 | |
| Number of reflections | 28906 | 2129 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 98.7 | 98.8 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1 M Bis-Tris pH 6.5, 29% PEG 3350, 0.22 mM CuSO4 |
