6GDV
Structure of CutA from Synechococcus elongatus PCC7942 complexed with Bis-Tris molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.590, 96.550, 48.900 |
| Unit cell angles | 90.00, 97.35, 90.00 |
Refinement procedure
| Resolution | 48.320 - 2.000 |
| R-factor | 0.19437 |
| Rwork | 0.192 |
| R-free | 0.25502 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1naq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.570 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.320 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rpim | 0.076 | 0.235 |
| Number of reflections | 20245 | 1498 |
| <I/σ(I)> | 5.6 | 1.6 |
| Completeness [%] | 94.2 | 93.2 |
| Redundancy | 2.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 0.1 M Bis-Tris pH 5.5, 0.2 M Li2SO4, 25% PEG 3350 |
