6GDU
Structure of CutA from Synechococcus elongatus PCC7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.149, 96.371, 48.977 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 48.190 - 1.750 |
| R-factor | 0.1988 |
| Rwork | 0.197 |
| R-free | 0.23223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gdv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.595 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.190 | 1.870 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.041 | 0.218 |
| Number of reflections | 32752 | 1772 |
| <I/σ(I)> | 11.2 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.5 |
| CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 294 | 0.1 M MIB buffer pH 4, 25% PEG 1500 |
