6GDT
Crystal structure of exo-glucosidase/glucosaminidase VC0615 from Vibrio Cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 234.899, 234.899, 129.421 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.330 - 3.170 |
| R-factor | 0.23742 |
| Rwork | 0.235 |
| R-free | 0.27978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h7l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 3.240 |
| High resolution limit [Å] | 3.170 | 3.170 |
| Rpim | 0.080 | 0.904 |
| Number of reflections | 69816 | 4464 |
| <I/σ(I)> | 8.4 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 10.8 |
| CC(1/2) | 0.997 | 0.322 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris pH 7.5, 5% PGA-LM, 8% PEG 20k |
