6GD3
Structure of HuR RRM3 in complex with RNA (UAUUUA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.740, 79.930, 54.870 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 6.040 - 1.350 |
| R-factor | 0.16573 |
| Rwork | 0.165 |
| R-free | 0.18859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gd1 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.330 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.040 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.072 | 0.670 |
| Number of reflections | 62913 | 4560 |
| <I/σ(I)> | 10.84 | 2.18 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 3.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M ammonium acetate, 0.01 M calcium chloride, 0.05 M sodium cacodylate pH 6.5 and 10 % (w/v) PEG 4000 |
