6GD2
Structure of HuR RRM3 in complex with RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-31 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.120, 80.480, 54.440 |
| Unit cell angles | 90.00, 90.69, 90.00 |
Refinement procedure
| Resolution | 8.500 - 1.900 |
| R-factor | 0.19428 |
| Rwork | 0.192 |
| R-free | 0.24513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gd1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.823 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.500 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.116 | 0.600 |
| Number of reflections | 23114 | |
| <I/σ(I)> | 9.09 | 2.38 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.5, 10% (w/v) PEG 8000 |
