6GCZ
Laminarin binding SusD-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-06-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 67.385, 69.416, 115.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.390 - 1.800 |
| R-factor | 0.16179 |
| Rwork | 0.160 |
| R-free | 0.19653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ihv |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.762 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.390 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 50993 | 7347 |
| <I/σ(I)> | 11.1 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.8 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | 0.2 M MgCl pH 7.8, 16% PEG 8000. |
