6GCO

Truncated FtsH from A. aeolicus in P312

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000000
Spacegroup name	P 3 1 2
Unit cell lengths	117.304, 117.304, 137.054
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	81.613 - 3.323
R-factor	0.2491
Rwork	0.240
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4ww0
RMSD bond length	0.003
RMSD bond angle	0.649
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	81.613	81.613	3.520
High resolution limit [Å]	3.320	9.900	3.320
Rmerge	0.099	0.025	1.071
Rmeas	0.104	0.027	1.131
Number of reflections	15949	656	2390
<I/σ(I)>	18.53	79.49	2.18
Completeness [%]	98.8	99.5	92.5
Redundancy	10.04	9.02	9.469
CC(1/2)	0.999	0.999	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.75	298	0.2 M KCl, 0.05 M HEPES/NaOH pH7.75, 33% pentaerythritol propoxylate 426 The affinity tag was cleaved by thrombin. This cleavage was just partial according to a SDS-PAGE analysis.