6GCN

Truncated FtsH from A. aeolicus in R32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000010
Spacegroup name	H 3 2
Unit cell lengths	197.550, 197.550, 323.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.586 - 2.949
R-factor	0.2142
Rwork	0.213
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	poorly refined structure in space group P312
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.586	60.586	3.030
High resolution limit [Å]	2.949	13.190	2.950
Rmerge	0.105	0.026	1.869
Rmeas	0.108	0.026	1.915
Number of reflections	51350	633	3763
<I/σ(I)>	24.73	93.58	1.92
Completeness [%]	100.0	98.9	99.8
Redundancy	20.588	18.839	20.875
CC(1/2)	1.000	1.000	0.716

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	278	60-65% Tacsimate pH 7.00, 0.1 M Bis-Tris-Propane/HCl pH 7.00