6GCB
Crystal structure of glutathione transferase Xi 3 from Trametes versicolor in complex with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97972 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.370, 72.370, 316.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.694 - 1.795 |
| R-factor | 0.1621 |
| Rwork | 0.161 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gca |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.977 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.694 | 1.840 |
| High resolution limit [Å] | 1.795 | 1.800 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 78924 | 4842 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 98.6 | |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 277 | 20% (w/v) polyethylene glycol 8,000, 0.1M pH 6.5 sodium cacodylate buffer and 0.2M magnesium acetate |
