6GBP
Crystal Structure of the oligomerization domain of VP35 from Ebola virus, mercury derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0080 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.421, 103.923, 186.287 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.490 |
| R-factor | 0.2237 |
| Rwork | 0.221 |
| R-free | 0.28110 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.986 |
| Data reduction software | XDS (VERSION Oct 15, 2015) |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | SHELXDE (2013/2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.570 | 46.570 | 3.820 |
| High resolution limit [Å] | 3.490 | 8.550 | 3.490 |
| Rmerge | 0.242 | 0.067 | 1.189 |
| Rmeas | 0.249 | 0.069 | 1.226 |
| Rpim | 0.060 | 0.017 | 0.294 |
| Total number of observations | 262316 | 16868 | 60835 |
| Number of reflections | 15726 | 1187 | 3595 |
| <I/σ(I)> | 10 | 33.1 | 2.9 |
| Completeness [%] | 99.2 | 99.4 | 96.8 |
| Redundancy | 16.7 | 14.2 | 16.9 |
| CC(1/2) | 0.998 | 0.999 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 296 | 50 mM HEPES-NaOH pH 7.5, 2.6 M Na-acetate |
