6GBI
Wnt signalling
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-01 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.980, 61.980, 68.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.970 - 1.250 |
| R-factor | 0.1623 |
| Rwork | 0.162 |
| R-free | 0.16460 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.164 |
| Data reduction software | DENZO |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.280 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 37608 | |
| <I/σ(I)> | 31.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 300 | 0.1 Tris-HCl 2M Ammonium Sulphate |
