6GBC
The Structure of variant R369A of the Mo-insertase domain Cnx1E from Arabidopsis thaliana in complex with AMP and molybdate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.840, 123.900, 131.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.771 - 1.590 |
| R-factor | 0.178 |
| Rwork | 0.177 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5g2r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS (Jun 1, 2017) |
| Data scaling software | STARANISO (1.10.9) |
| Phasing software | PHASER (2.8.1) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.771 | 1.728 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.055 | 1.433 |
| Rmeas | 0.060 | 1.544 |
| Rpim | 0.022 | 0.573 |
| Number of reflections | 51433 | 2572 |
| <I/σ(I)> | 21.1 | |
| Completeness [%] | 70.9 | 16.2 |
| Redundancy | 13.4 | 14 |
| CC(1/2) | 0.999 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M imidazole, 0.1 M MES pH 6.5; 0.06 M sodium nitrate, 0.06 M sodium phosphate, 0.06 M ammonium sulfate; 20 % (v/v) PEG 500 MME, 10 % (w/v) PEG 8000; 0.015 M sodium molybdate |
