6GBA
A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - M49A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-17 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.880, 30.931, 49.132 |
| Unit cell angles | 90.00, 112.93, 90.00 |
Refinement procedure
| Resolution | 28.974 - 1.900 |
| R-factor | 0.1796 |
| Rwork | 0.177 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kuk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.651 |
| Rmeas | 0.079 | 0.746 |
| Number of reflections | 9733 | 627 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 97.5 | 98.2 |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.999 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292.15 | 0.1 M Tris-HCl, 30 % PEG 6000 |
