6GAX

The Structure of variant K294A of the Mo-insertase domain Cnx1E from Arabidopsis thaliana in complex with AMP and molybdate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	65.220, 122.130, 131.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.910 - 1.770
R-factor	0.187
Rwork	0.186
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5g2r
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	XDS (Jun 1, 2017)
Data scaling software	STARANISO (1.10.9)
Phasing software	PHASER (2.8.1)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.545	1.937
High resolution limit [Å]	1.768	1.768
Rmerge	0.103	1.495
Rmeas	0.111	1.612
Rpim	0.042	0.599
Number of reflections	36842	1843
<I/σ(I)>	12.8
Completeness [%]	71.2	15
Redundancy	13.5	13.6
CC(1/2)	0.990	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.015 M sodium molybdate