6GAV
Extremely 'open' clamp structure of DNA gyrase: role of the Corynebacteriales GyrB specific insert
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.506, 157.617, 103.504 |
| Unit cell angles | 90.00, 98.11, 90.00 |
Refinement procedure
| Resolution | 46.150 - 2.600 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3IFZ 3IG0 3ZKD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.150 | |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.104 | 1.253 |
| Number of reflections | 94824 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.38 | |
| CC(1/2) | 0.995 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 100 mM Sodium Acetate 100 mM MES pH 6.5 26% PEG 400 25% EG 10 mM MgCl2 |
