6GAP
Crystal structure of the T3D reovirus sigma1 coiled coil tail and body
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 350.710, 41.530, 63.320 |
| Unit cell angles | 90.00, 94.98, 90.00 |
Refinement procedure
| Resolution | 49.150 - 2.150 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gaj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.150 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmeas | 0.127 | 0.995 |
| Number of reflections | 50259 | 3674 |
| <I/σ(I)> | 13.7 | 2.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.999 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 40% MPD, 0.1M Tris pH 8.0, 3% (w/v) 1,6-diaminohexane |
