6GAO
Crystal structure of the T1L reovirus sigma1 coiled coil tail and body
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.650, 33.990, 123.480 |
| Unit cell angles | 90.00, 101.38, 90.00 |
Refinement procedure
| Resolution | 42.194 - 2.101 |
| R-factor | 0.1915 |
| Rwork | 0.189 |
| R-free | 0.24860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gaj |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.068 |
| Data reduction software | X-Area |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.194 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.169 | 0.917 |
| Number of reflections | 35260 | 2552 |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.3 | 5.6 |
| CC(1/2) | 0.997 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 35% (v/v) 1,4-dioxane, |
