6GAK
Crystal structure of the T1L reovirus sigma1 coiled coil tail (chloride)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.370, 37.190, 94.940 |
| Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
| Resolution | 20.850 - 1.430 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gaj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.660 | 1.520 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmeas | 0.123 | 0.714 |
| Number of reflections | 67336 | 10431 |
| <I/σ(I)> | 11 | 2.1 |
| Completeness [%] | 99.3 | 96.1 |
| Redundancy | 6.6 | 6.1 |
| CC(1/2) | 0.999 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 277 | 20% PEG8000, 0.1M CHAPS pH 10.5, 0.2M NaCl |
