6GAJ
Crystal structure of the T1L reovirus sigma1 coiled coil tail (iodide)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.700, 37.590, 89.720 |
| Unit cell angles | 90.00, 100.53, 90.00 |
Refinement procedure
| Resolution | 48.730 - 1.350 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20260 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmeas | 0.109 | 0.876 |
| Number of reflections | 76273 | 5563 |
| <I/σ(I)> | 12 | 1.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6 |
| CC(1/2) | 0.999 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 18% (v/v) PEG8000, 0.1M CAPS pH 9.5, 0.2M NaI |
