6GA3
Bacteriorhodopsin, 33 ms state, ensemble refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-07-26 |
| Detector | CS-PAD CXI-2 |
| Wavelength(s) | 1.26 |
| Spacegroup name | P 63 |
| Unit cell lengths | 62.100, 62.100, 109.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.545 - 2.100 |
| R-factor | 0.1946 |
| Rwork | 0.192 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b6v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.121 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 13984 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 144 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.6 | 293 | 32% (w/v) PEG 2000, 0.1 M K2HPO4 /NaH2PO4 |
