6G9Z

Crystal structure of human histidine triad nucleotide-binding protein 1 (hHINT1) crystallized at P212121 space group, with visible extended fragment of N-terminus

Replaces:  5O8G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	P 21 21 21
Unit cell lengths	46.075, 63.379, 76.836
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.520 - 1.430
R-factor	0.2263
Rwork	0.224
R-free	0.27726
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw2
RMSD bond length	0.016
RMSD bond angle	1.812
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.520	1.510
High resolution limit [Å]	1.430	1.430
Rmerge	0.137	0.703
Rmeas	0.161	0.821
Number of reflections	40954	6294
<I/σ(I)>	8.78	1.96
Completeness [%]	96.1	92.7
Redundancy	3.77	3.74
CC(1/2)	0.994	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	281	25% PEG 1500, 0.1 M MMT Buffer pH 7.0