6G9R
Murine class I major histocompatibility complex H-2 Db in complex with self-antigen derived from dopamine monooxygenase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.245, 123.597, 99.262 |
| Unit cell angles | 90.00, 103.07, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.700 |
| R-factor | 0.23562 |
| Rwork | 0.234 |
| R-free | 0.26441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.522 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.059 | 0.427 |
| Rpim | 0.059 | |
| Number of reflections | 56263 | 11115 |
| <I/σ(I)> | 8.2 | 1.5 |
| Completeness [%] | 94.5 | 95.9 |
| Redundancy | 2.4 | |
| CC(1/2) | 0.998 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.5-2.0M ammonium sulfate 0.1M TRIS HCL, pH 8.0 |
