6G9C
Crystal structure of immunomodulatory active chitinase from Trichuris suis, TsES1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 196.083, 52.613, 93.450 |
Unit cell angles | 90.00, 108.79, 90.00 |
Refinement procedure
Resolution | 46.115 - 1.740 |
R-factor | 0.1757 |
Rwork | 0.175 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hbf |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.410 | 46.410 | 1.770 |
High resolution limit [Å] | 1.740 | 9.530 | 1.740 |
Rmerge | 0.084 | 0.024 | 2.427 |
Rmeas | 0.093 | 0.026 | 2.685 |
Rpim | 0.038 | 0.011 | 1.129 |
Number of reflections | 92411 | 622 | 4506 |
<I/σ(I)> | 10.9 | ||
Completeness [%] | 99.5 | 98.7 | 99.3 |
Redundancy | 5.6 | 5.2 | 5.4 |
CC(1/2) | 0.999 | 0.998 | 0.322 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 293 | 100mM Bis_Tris, 200mM Ammonium sulfate, 25% PEG 3350 |