6G96

Crystal structure of TacT3 (tRNA acetylating toxin) from Salmonella

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 21 21 21
Unit cell lengths	64.786, 71.202, 86.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.925 - 1.477
R-factor	0.209747103769
Rwork	0.208
R-free	0.23534
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fvj
RMSD bond length	0.006
RMSD bond angle	1.058
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.310	1.500
High resolution limit [Å]	1.470	1.480
Rpim	0.094
Number of reflections	67592	3298
<I/σ(I)>	8.7	2.2
Completeness [%]	99.8	100
Redundancy	6.6
CC(1/2)		0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1M Bicine 10% PEG 6000 0.25M NaCl