6G85

Structure of Cdc14 bound to CBK1 PxL motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	76.154, 97.408, 129.176
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.995 - 1.528
R-factor	0.1834
Rwork	0.182
R-free	0.20890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohc
RMSD bond length	0.020
RMSD bond angle	1.307
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.700	1.580
High resolution limit [Å]	1.520	1.520
Rmerge	0.050	0.850
Rpim	0.020	0.450
Number of reflections	145196	64686
<I/σ(I)>	10.6	1.22
Completeness [%]	97.3	92.9
Redundancy	5.2	4.6
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1.5% PEG 6000 and 0.1 M MES pH 6.0