6G82
Serum paraoxonase-1 by directed evolution with the L69S/H115W/F222S mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-07 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.9334 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 98.135, 98.135, 139.189 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.569 - 2.401 |
R-factor | 0.1903 |
Rwork | 0.188 |
R-free | 0.22260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hho |
RMSD bond length | 0.007 |
RMSD bond angle | 0.850 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.569 | 2.487 |
High resolution limit [Å] | 2.401 | 2.401 |
Rmerge | 0.115 | 0.514 |
Number of reflections | 27165 | |
<I/σ(I)> | 36.17 | |
Completeness [%] | 99.7 | |
Redundancy | 13.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Bis-Tris Propane pH 6.5, 0.2M Sodium bromide and 20% PEG 3350. |