6G7E

Crystal structure of Chaetomium thermophilum Mot1 (E1434Q, 1837-1886 deletion mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97916
Spacegroup name	P 1 21 1
Unit cell lengths	93.134, 96.850, 129.640
Unit cell angles	90.00, 97.55, 90.00

Refinement procedure

Resolution	48.697 - 3.213
R-factor	0.1933
Rwork	0.191
R-free	0.23790
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	XDS (May 1, 2016 (BUILT=20160617))
Data scaling software	XSCALE (May 1, 2016 (BUILT=20160617))
Phasing software	PHENIX (1.10.1-2155)
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.690	3.300
High resolution limit [Å]	3.210	3.210
Rmerge	0.130	0.810
Rmeas	0.140	0.890
Number of reflections	36422
<I/σ(I)>	11.8	2.6
Completeness [%]	97.1	68.8
Redundancy	6.6	5.8
CC(1/2)	0.990	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2 M ammonium acetate, 0.1 M Tris pH 8.9, 12% PEG 3350