6G7C
Nt2-CTD domains of the TssA component from the type VI secretion system of Aeromonas hydrophila.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.034, 202.076, 139.013 |
| Unit cell angles | 90.00, 90.72, 90.00 |
Refinement procedure
| Resolution | 202.080 - 3.130 |
| R-factor | 0.25856 |
| Rwork | 0.255 |
| R-free | 0.32758 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.599 |
| Data reduction software | xia2 |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 202.080 | 3.210 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Rmerge | 0.113 | |
| Rpim | 0.077 | |
| Number of reflections | 67977 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 280 | 0.2 M sodium acetate, 0.1 M sodium citrate (pH 5.5), and 10 % (w/v) PEG 4000 |
